AGRO 126

Biomass-to-ethanol conversion: Strategies for developing flexible biorefineries

William J. Orts, Kevin M. Holtman, Gregory M. Glenn, gmg@pw.usda.gov, Richard Offeman, roffeman@pw.usda.gov, George H. Robertson, grobertson@pw.usda.gov, Syed H. Imam, simam@pw.usda.gov, and Dominic W. S. Wong, dwsw@pw.usda.gov. Western Regional Research Center, USDA-Agricultural Research Service, 800 Buchanan St., Albany, CA 94710-1100
In order for ethanol production to be viable in the western states, a wide range of feedstocks will need to be utilized which will require flexibility in the processing capabilities. For example, California has a limited supply of corn starch, but is the leading agricultural producer of more than 30 distinctly different crops, ranging from garlic, to artichokes, strawberries, walnuts, and grapes. The USDA-ARS has addressed the need for flexible processing of lignocellulosic material via a targeted program aimed at creating the athletic biorefinery, whereby, biomass from a wide array of feedstocks is converted to ethanol within the same plant throughout all seasons. This summary will focus on our strategies to meet this target, including (1) new enzymes and technologies for cellulose-to-ethanol capabilities via directed evolution of microbes, (2) novel separation engineering for ethanol and bioproduct isolation, (3) application of bioproducts, biobased plastics, and co-product utilization, and (4) crop improvement via plant molecular biology. Specific research results will be presented on such areas as cold starch hydrolysis for reducing energy costs during ethanol production, microbial screening methods for improving enzyme specificity and yield, and engineering considerations in developing the flexible, athletic biorefinery. One example of particular note is development of a biomass-to-ethanol pilot plant utilizing a mixture of municipal solids waste (MSW) and ag-derived biomass.